Part:BBa_K4516006

pcDNA3.1-backbone

pcDNA3.1-backbone

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 312
  Illegal PstI site found at 1647
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NheI site found at 292
  Illegal PstI site found at 1647
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 12
  Illegal XhoI site found at 318
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 312
  Illegal PstI site found at 1647
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 312
  Illegal PstI site found at 1647
  Illegal NgoMIV site found at 757
  Illegal NgoMIV site found at 2098
  Illegal NgoMIV site found at 2381
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 279
  Illegal BsaI.rc site found at 3906
  Illegal SapI site found at 2826
  Illegal SapI.rc site found at 1947
  Illegal SapI.rc site found at 2157